NOTES TO CONTRIBUTORS
INSTRUCTIONS TO AUTHORS
ONLINE SUBMISSION
EDITORIAL BOARD
Editor-in-Chief Naoyuki TANIGUCHI (Suita)
Editors
| JB Reviews |
Atsushi MIYAJIMA (Tokyo) |
| Biochemistry |
Akemi SUZUKI (Wako) |
|
Seiki KURAMITSU (Toyonaka) |
| Molecular Biology |
Yoshihide TSUJIMOTO (Suita) |
| Cell |
Kohei MIYAZONO (Tokyo) |
| Biotechnology |
Masahiro IWAKURA (Tsukuba)
|
Managing Editors
| Masahiro NISHIJIMA (Tokyo) |
Tanihiro YOSHIMOTO (Kanazawa) |
Associate Editors
| JB Reviews |
Akiyoshi FUKAMIZU (Tsukuba) |
Tatsuro IRIMURA (Tokyo) |
|
Hisao MASAI (Tokyo) |
Toru NAKANO (Suita) |
| Biochemistry |
Tamao ENDO (Tokyo) |
Harumi FUKADA (Sakai) |
|
Toshiharu HASE (Suita) |
Koichi HONKE (Nankoku) |
|
Takashi IZUMI (Maebashi) |
Nobuo KAMIYA (Osaka) |
|
Hiroshi KIDO (Tokushima) |
Makoto KIMURA (Fukuoka) |
|
Takahito KONDO (Nagasaki) |
Takeshi NISHINO (Tokyo) |
|
Masato UMEDA (Uji) |
Yuriko YAMAGATA (Kumamoto) |
|
|
|
| Molecular Biology |
Kenji KOHNO (Ikoma) |
Yusaku NAKABEPPU (Fukuoka) |
|
Yoshinobu NAKANISHI (Kanazawa) |
Shoko NISHIHARA (Hachioji) |
|
Shoji TAJIMA (Suita) |
|
| Cell |
Kiyoko FUKAMI (Hachioji) |
Yutaka HATA (Tokyo) |
|
Shigeki HIGASHIYAMA (Ehime) |
Masaki INAGAKI (Nagoya) |
|
Akira KIKUCHI (Hiroshima) |
Takashi YOKOTA (Kanazawa) |
| Biotechnology |
Keiko MIZUTA (Higashihiroshima) |
Fumio MIZUTANI (Sapporo) |
|
Takahiro OCHIYA (Tokyo) |
Kiyotaka SHIBA (Tokyo) |
|
Kouhei TSUMOTO (Kashiwa) |
|
Advisory Board
Biochemistry
| Satoko AKASHI (Yokohama) |
Hideo AKUTSU (Suita) |
Kiyoshi FUKUI (Tokushima) |
| Rudolf GEYER(Giessen) |
Osami HABUCHI (Kariya) |
Fumio HAYASHI (Kobe) |
| Masao IKEDA-SAITO (Sendai) |
Shinobu IMAJOH-OHMI (Tokyo) |
Hiroaki KATO (Kyoto) |
| Tatsuya MAEDA (Tokyo) |
Kei MARUYAMA (Saitama) |
Takashi MATOZAKI (Maebashi) (Tokyo) |
| Teru OGURA (Kumamoto) |
Mamoru SATO (Yokohama) |
Yoshitsugu SHIRO (Hyogo) |
| Koji SUZUKI (Tsu) |
Yasuhiro TAKAHASHI (Toyonaka) |
Motonari TSUBAKI (Kobe) |
| Soichi WAKATSUKI (Tsukuba) |
Takehiko YOKOMIZO (Tokyo) |
|
Molecular Biology
| David S. GILMOUR (University Park) |
Kazuhiko IGARASHI (Hiroshima) |
Dongchon KANG (Fukuoka) |
| Makoto KOBAYASHI (Tsukuba) |
Kenji KUROKAWA (Tokyo) |
Mitsuyoshi NAKAO (Kumamoto) |
| Yoshimichi NAKATSU (Fukuoka) |
Hiroshi NISHINA (Tokyo) |
Yoshiaki OHKUMA (Suita) |
| Akira SHINOHARA (Suita) |
Toshiki TSURIMOTO (Fukuoka) |
Tadashi UEDA (Fukuoka) |
| Chieko YOKOYAMA (Suita) |
|
Cell
| Hiroshi ITOH (Ikoma) |
Shinji KAMIMURA (Tokyo) |
Daisuke KOHDA (Fukuoka ) |
| Takao NAKATA (Tokyo) |
Keiichi NAKAYAMA (Fukuoka) |
Hitoshi NIWA (Kobe) |
| Masato OKADA (Suita) |
Junichi TAKAGI (Suita) |
Yuji YAMANASHI (Tokyo) |
| Marino ZERIAL (Dresden) |
|
|
|
|
|
|
|
|
|
|
|
Biotechnology
| Kazunori AOKI (Tokyo) |
Kazuo HARADA (Koganei) |
Izuho HATADA (Maebashi) |
| Shinya HONDA (Tsukuba) |
Kenji KANO (Kyoto) |
Tomokazu MATSUE (Sendai) |
| Osamu NIWA (Atsugi) |
Hiroki SASAKI (Tokyo) |
Hiroaki SUGA (Tokyo) |
| Hiroshi TAKAGI (Fukui) |
Hideki YORIMITSU (Kyoto) |
Noboru YUMOTO (Ikeda) |
|
This JOURNAL, devoted to publication of original papers in the fields of biochemistry,
molecular biology, cell, and biotechnology, was founded in 1922.
The JOURNAL is published monthly, with two volumes per annum.
All correspondences concerning this JOURNAL should be addressed to: The Japanese
Biochemical Society, Ishikawa Building-3f, 25-16, Hongo 5-chome, Bunkyo-ku, Tokyo
113-0033, Japan. Tel. + 81-3-3815-1913, Fax. +81-3-3815-1934
Subscription
The price for a subscription per year (two volumes) is US$385.00 (Print and Online),
US$315.00 (Online Only), or US$350.00 (Print Only).
Remittance should be made preferably by check or draft
payable to the Japanese Biochemical Society.
Subscriptions from abroad should be paid to the agencies listed below.
Agencies
| Oxford University Press. Journals Subscription Department Great Clarendon Street Oxford, OX2 6DP, UK |
Japan Publications Trading Co., Ltd., P.O. Box 5030, Tokyo International, 2-1,
Sarugaku-cho 1-chome, Chiyoda-ku, Tokyo 101-0064, Japan |
Maruzen Co., Ltd., P.O. Box 5050, Tokyo International, 100-3199, Japan |
All back issues of the Journal of Biochemistry from Volume 1, 1922, are
available from our agency: TOA BOOK EXPORTS, INC., Ikebukuro 4-13-4, Toshima-ku, Tokyo
171-0014, Japan. Tel. +81-3-3985-4701, Fax. +81-3-3985-4703
Copyright © 2005, by the Japanese Biochemical Society
Notice on photocopying With the exception of cases permitted by the Copyrights Law,
such as the photocopying services provided by the libraries duly designated by government
ordinance and photocopying for lectures by teachers at educational institutions, the
photocopying of any part of this publication without explicit consent of the relevant
author is a violation of the law. To legally photocopy any part of this publication, the
person wishing to photocopy, or the corporation or organization employing such person, is
recommended to enter into a comprehensive copyright approval agreement with the following
trustee of copyrights from the authors:
(Except in the U.S.A.) The Copyright Council of the Academic Societies, 41-6, Akasaka
9-chome, Minato-ku, Tokyo 107-0052, Japan. Phone: 3-3475-4621, Fax: 3-3403-1738
(In U.S.A.) Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, MA 01923,
U.S.A. Phone: 508-750-8400, Fax: 508-750-4744
[JB Homepage]
The
JOURNAL OF BIOCHEMISTRY publishes contributions written in English and submitted in the
form of (1) Regular paper or (2) Rapid communication, covering materials requiring prompt
publication.
The
submission of a manuscript implies that the work has not been published previously, that
it is not under consideration for publication elsewhere, and that if it is accepted for
publication, the author(s) will transfer the copyright to the Japanese Biochemical
Society.
Manuscripts
should be succinct. No length limit is set for a Regular paper, whereas a Rapid
communication must not exceed 3.5 printed pages. A charge of 700 yen is made for every
printed page of a Regular paper and 7,200 yen per page for a Rapid communication.
The
manuscript should be typewritten and double-spaced, and the pages must be appropriately
numbered. The submission of four manuscript copies is requested. They should be sent to
The Japanese Biochemical Society, Ishikawa Building-3f, 25-16, Hongo 5-chome, Bunkyo-ku,
Tokyo 113-0033, Japan.
The JB-Online manuscript submission and reviewing system is
now available. We strongly recommend you to use this system. It greatly reduces overhead
time and the usual extensive manual procedures required by traditional reviewing and
printing processes. Thus the prompt publication of your manuscrip may be expected. For
further details online: Instructions for Online Submission. Notice: Paper manuscripts may
be submitted for a limited time.
Reprints can be purchased, in lots of 50 copies, at cost prices. The orders should be submitted with the returned proof.
When submitting manuscripts, authors are asked to select one of the following four
categories and its topic under which the submission should be reviewed. Please indicate
this selection on the title page of the manuscript. Authors may suggest names and
addresses of persons suitable for reviewing their submitted works.
Fields and topics to be selected:
| Fields |
Topics |
| Biochemistry |
Biochemistry General |
|
Protein Structure |
|
Protein Interaction and Recognition |
|
Biomolecular Structures |
|
Nucleic Acid and Peptide Biochemistry |
|
Glycobiology and Carbohydrate Biochemistry |
|
Lipid Biochemistry |
|
Enzymology |
|
Enzyme Inhibitors |
|
Biochemistry of Proteolysis |
|
Metabolism and Bioenergetics |
|
Reactive Oxygen and Nitrogen Species |
|
Biochemistry in Cell Membranes |
|
Biochemistry in Diseases and Aging |
|
Neurochemistry |
|
Immunochemistry |
|
Physiological Chemistry |
|
Biochemical Pharmacology |
|
Analytical Biochemistry |
| Molecular Biology: |
Molecular Biology General |
|
Genes and Other Genetic Materials |
|
Replication and Recombination |
|
Gene Expression |
|
Protein Synthesis |
|
DNAProtein Interaction |
|
RNA Processing |
|
Genetic Engineering |
|
Genetic Diseases |
|
Molecular Genetics |
|
Molecular Evolution |
|
Bioinformatics |
| Cell: |
Cell General |
|
Biomembranes, Organelles, and Protein Sorting |
|
Muscles |
|
Cytoskeleton, Cell Motility, and Cell Shape |
|
Extracellular Matrices and Cell Adhesion Molecules |
|
Cell Cycle |
|
Receptors and Signal Transduction |
|
Stress Proteins and Molecular Chaperones |
|
Cell Death |
|
Differentiation, Development, and Aging |
|
Neurobiology |
|
Tumor and Immunology |
| Biotechnology: |
Biotechnology General |
|
Biomimetic Chemistry |
|
Biomaterials |
|
Bioactive Substances |
|
Synthetic Peptides and Oligonucleotides |
|
Gene and Protein Engineeing |
|
RNA Technology |
|
Glycotechnology |
|
Immunological Engineering |
|
Cell and Tissue Engineering |
|
Transgenic Technology |
|
Gene Delivery Systems |
|
Drug Delivery Systems |
|
Biosensor and Bioelectronics |
|
New Devices in Biotechnology |
|
Environmental Technology |
"Instructions to Authors" are printed in the January and July issues of this
JOURNAL every year. Please conform to these instructions when submitting manuscripts.
Reprint of "Instructions to Authors" is available.
New Announcement (Limited Publication)
JOURNAL OF BIOCHEMISTRY, GENERAL CONTENTS
| Volume 37-66 (1950-1969) Cloth Bound 700 page, including Author and Subject Index US
$90.00 |
Volume 67-98 (1970-1985) Cloth Bound 1000 page, including Author and Subject Index US
$120.00 |
Now available through: TOA BOOK EXPORTS, INC.,
Ikebukuro 413-4, Toshima-ku, Tokyo 171-0014, Japan. [JB
Homepage]
INSTRUCTIONS TO AUTHORS
The Journal of Biochemistry
Published by
The Japanese Biochemical Society (2005)
The Journal of Biochemistry publishes the results of original
research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written
in English in the form of Regular Papers or Rapid Communications. A Rapid Communication
is not a preliminary note, but it is, though brief, a complete and final publication.
The materials described in Rapid Communications should not be included in a later paper.
The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial
Board. The submission of a manuscript implies that the work described has not been
published previously, that it is not under consideration for publication elsewhere, and
that if it is accepted for publication, the author(s) will transfer the copyright to
the Japanese Biochemical Society. The JB-Online
manuscript submission and reviewing system is now available. We strongly recommend that
you use the online system. It greatly reduces overhead time and the usual extensive manual
procedures required by traditional reviewing and printing processes. Thus the prompt
publication of your manuscript may be expected. For further details online: Instructions for
Online Submission. Notice: Paper manuscripts may be submitted for a limited
time.
I. GENERAL INFORMATION
Manuscripts should be submitted in quadruplicate (one original and three clear
copies) to:
The Editorial Office
The Japanese Biochemical Society
Ishikawa Building-3f
25-16, Hongo 5-chome, Bunkyo-ku
Tokyo 113-0033, Japan
Tel. +81-3-3815-1913
Fax. +81-3-3815-1934
On submission of a paper, authors are requested to select one of the following four
fields and its topic, under which the submitted paper should be reviewed, and to indicate
their selection on the title page of the manuscript. Fields to be selected:
Biochemistry
Molecular Biology
Cell
Biotechnology
Topics to be selected:
Biochemistry: Biochemistry General; Protein Structure; Protein Interaction and
Recognition; Biomolecular Structures; Nucleic Acid and Peptide Biochemistry; Glycobiology
and Carbohydrate Biochemistry; Lipid Biochemistry; Enzymology; Enzyme Inhibitors;
Biochemistry of Proteolysis; Metabolism and Bioenergetics; Reactive Oxygen and Nitrogen
Species; Biochemistry in Cell Membranes; Biochemistry in Diseases and Aging;
Neurochemistry; Immunochemistry; Physiological Chemistry; Biochemical Pharmacology;
Analytical Biochemistry
Molecular Biology: Molecular Biology General; Genes and Other Genetic Materials;
Replication and Recombination; Gene Expression; Protein Synthesis; DNA-Protein
Interaction; RNA Processing; Genetic Engineering; Genetic Diseases; Molecular Genetics;
Molecular Evolution; Bioinformatics
Cell: Cell General; Biomembranes, Organelles, and Protein Sorting; Muscles;
Cytoskeleton, Cell Motility, and Cell Shape; Extracellular Matrices and Cell Adhesion
Molecules; Cell Cycle; Receptors and Signal Transduction; Stress Proteins and Molecular
Chaperones; Cell Death; Differentiation, Development, and Aging; Neurobiology; Tumor and
Immunology
Biotechnology: Biotechnology General; Biomimetic Chemistry; Biomaterials; Bioactive
Substances; Synthetic Peptides and Oligonucleotides; Gene and Protein Engineering; RNA
Technology; Glycotechnology; Immunological Engineering; Cell and Tissue Engineering;
Transgenic Technology; Gene Delivery Systems; Drug Delivery Systems; Biosensor and
Bioelectronics; New Devices in Biotechnology; Environmental Technology
No definite limit of length is set for a Regular Paper, but all manuscripts
should be as concise as possible. A concise well-written paper will usually reduce the
time required for review and tends to be published more rapidly. A Rapid Communication
should not exceed an equivalent of 3.5 printed pages including the spaces required for
figures, tables, and references. In estimating this limit, note that one single printed
page is approximately 3.5 pages of a double-spaced type-written manuscript.
A manuscript describing primary structures of biological macromolecules (proteins and
nucleic acids) without enough data for their deductions within the limited page space is
not acceptable as a Rapid Communication. In the case of a Rapid Communication, the
author should submit two copies of a free style letter describing the urgency or necessity
for the rapid publication. [JB Homepage]
II. REVIEW PROCESS
Manuscript will be sent to at least two referees for evaluation. The JOURNAL always
attempts to minimize the potential for conflict of interest in the review of manuscripts.
Therefore, authors may request that a specific individual with a possible conflict of
interest not be involved in reviewing the manuscript. Authors may suggest the names and
addresses of a few potential reviewers. The Editors and Associate Editors will be guided
but not necessarily bound by these suggestions.
Contributors will receive a letter from one of the Editors or Associate Editors stating
whether their manuscript is acceptable. Revised manuscripts, and correspondence concerning
manuscripts, should be sent directly to the relevant Editor at the address indicated on
the letter. Revised papers will be considered as newly submitted papers if they are not
resubmitted within 2 months for no justifiable reason. The manuscript may be sent to a
member of the English revisers associated with the Society to correct English grammar and
syntax depending on the Editor's judgment before the final acceptance. Handling of
manuscripts is free of charge. Manuscripts, if accepted, will be published only after
agreement by the author(s) to pay the costs of publication including page charges (see "notes to contributors" in recent issues). Authors should
provide "materials for the preparation of indices" upon request from the Editor.
Alteration in galley proofs, other than the correction of printer's errors, are not
granted, except when the Editor admits inevitable addition of a brief note in proofs at
the author's expense. Galley proofs corrected by authors should be returned to the printer
by a designated date. Otherwise, the Editor reserves the right of proofreading.
Illustrations, photographs, electron micrographs, color plates, and other special
illustrations will be reproduced at the author's expense at cost prices. The list of these
cost prices will be sent to the author after the final decision.
Reprints can be purchased, in lots of 50 copies, at cost prices. The orders should be submitted with the returned proof.
When the manuscript is rejected, only the artwork (Tables and Figures) will be returned
to the author. The copies will be discarded by the Editorial office.
The members of the Editorial Board use the following
guidelines to assist them in making editorial decisions. To inform prospective authors of
our criteria, the guidelines are listed below, but please note that these are only
guidelines. (i) Is the subject suitable for publication in the Journal of Biochemistry?
(ii) Is it an original contribution? (iii) Is it a complete and final paper? (iv) Is it
clearly presented? (v) Are the summary and title informative? Do they reflect the contents
of the paper? (vi) Are the appropriate key words given? (vii) Does the introduction
contain statements sufficient to explain the aim of the work? (viii) Are the methods
sound? (ix) Are the results relevant and sufficient? (x) Are the illustrations and tables
necessary and acceptable? (xi) Are the interpretations and conclusions justified by the
data? (xii) Are the references adequate; are all of them necessary? Does the list of
references contain all the information?
In general, the Journal of Biochemistry will not publish papers that are: (i)
Merely confirmatory or descriptive as to the presence of a well-known process in tissues
or organisms not previously studied. (ii) Not novel enough: purification of an enzyme or
sequencing of a protein or nucleic acid which has already been reported for another
species or organ, unless the manuscript includes novel findings or is of biological
significance. (iii) Too preliminary or incomplete: incomplete amino acid or nucleotide
sequences, incomplete structures of natural compounds, incomplete NMR or other
spectroscopic assignments, etc. (iv) Deals only with the description of a new
method or the preparation of a reagent such as a monoclonal antibody, unless it is novel
or represents a substantial improvement. (v) Too specialized in areas outside the scope of
the Journal of Biochemistry. (vi) Just negative. [JB
Homepage]
III. FORM AND STYLE OF
MANUSCRIPT
Manuscripts should conform to the style and usage of the Journal as exemplified in
current issues. They should be typed on A4 form (21 x 29.7 cm or 21.6 x 28 cm) paper with
double-spacing throughout. Separate sheets should be used for the following: (1) title page(s), (2) summary, (3) text,
(4) footnote(s) to the text, (5) references, (6) table(s), (7) legend(s) to figure(s),
(8) figures or other subsidiary matters. The manuscripts
should be arranged in the order indicated above and all sheets should be numbered in
succession except the figure(s), the title page being page 1. Indicate the appropriate
location in the text of the tables, figures, and other subsidiary materials by marginal
notes. Latin words should be italicized (for example: in vitro, i.e., etc., per se).
Footnote(s) to the title, author's name(s), and affiliation(s) should appear on the title
page. Footnotes to the text should be typed on a separate sheet. All footnotes should be
numbered in succession with superscript, arabic numerals, starting from the title page
footnote(s). Footnotes to tables should be identified with superscript lower case (a, b, etc.),
and placed at the bottom of the table.
IV. ORGANIZATION OF MANUSCRIPT
A desirable plan for the organization of a Regular Paper is as follows: (a)
SUMMARY, (b) INTRODUCTION with no heading, (c) EXPERIMENTAL PROCEDURES or MATERIALS
AND METHODS, (d) RESULTS, (e) DISCUSSION, (f) REFERENCES. In some cases, presentation
will be clearer and more effective if the author combines some of these section. For a Rapid
Communication, a brief summary is requested, but headings and subheadings should be
omitted.
1. Title Page(s)
Provide a title page(s), containing the following items.
- The form of the paper (Regular Paper or Rapid Communication) and the field under which
the paper is to be reviewed.
- Title. The title should be informative and as short as is consistent with clarity. The
title should not include chemical formulae or arbitrary abbreviations, but chemical
symbols may be used to indicate the structures of isotopically labeled compounds. The
numbering of parts in a series of papers is not permitted, but titles and subtitles may be
used if necessary.
- By-line. List full names of all authors. A footnote reference(s) to an author(s),
indicating a change of address, should be given on the title-page.
- From-line. List the institution(s) in which the work was carried out, and the Zip Code,
if available.
- Running title. Provide a short running title of less than 60 strokes. It should be as
informative as possible.
- The name, complete mailing address, telephone number, and (if accessible) Fax number and
E mail address of the person to whom correspondence should be sent. To expedite the
review, much of the journal's correspondence will be by Fax, unless the authors request
use of regular mail when submitting the manuscript. The Japanese author(s) must also list
in Japanese the name and address of the person who is in charge of proofreading.
- Abbreviations. Non-standard abbreviations (see Section IX-6, 7, and 8) should be
defined, even if they are known to those familiar with the field. List all non-standard
abbreviations used in the paper in alphabetical order in a footnote on the title page.
2. Summary
(1) Every paper should have summary. The summary should be concisely written in less
than 200 words. Summaries of Rapid Communications should be limited to 100 words. The
summary should briefly present the problem, suggest the scope of the work and the plan of
experiments, mention significant data and state major findings and conclusions. Avoid
statements such as "The significance of these results is discussed" that do not
help the reader. The summary should be intelligible to the nonspecialist as well as the
specialist in your field, and hence should avoid specialized terms and abbreviations.
(2) Key words. Provide five key words identifying the nature of the subject matter alphabetically
in the last part of the summary.
3. Introduction
The text of a Regular Paper should begin with a short introduction with no heading.
This should state the reasons for performing the work, with brief reference to previous
work on the subject. Avoid giving an extensive review of the literature.
4. Methods, Results, and Discussion
The arrangement of the paper after the introduction is not fixed. The author may separate
sections with italicized subheadings.
The Experimental Procedures or Materials and Methods should give sufficient
details to enable the reader to repeat your work exactly, if necessary. The necessity
for conciseness should not lead to omission of important experimental details. Refer
to previously published procedures employed by citation of both the original description
and pertinent published modifications, and do not include extensive description unless
they present substantially new modifications. Combination of the Results and Discussion in
a single section sometimes gives a clearer and more compact presentation.
5. References
References cited in the text should be numbered in parentheses with italicized Arabic
numerals in order of appearance. References to "unpublished experiments" and
"personal communications" should appear parenthetically in the text following
the name(s) of the source of information [(Yamada, T., personal communication), (Suzuki,
M. and Yoshida, M., unpublished observations) etc.]. Be sure to verify the wording
of any personal communication with the person who supplied the information and get his
approval for the use of his name in connection with the quoted information. All references
should be listed in numerical order typed double-spaced on a separate sheet under the
heading REFERENCES. Please note the following examples.
(1) For a journal article:
- Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with the
chain-termination inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467
(2) For a chapter in an edited book:
- Messing, J. (1983) New M13 vectors for cloning in Methods in Enzymology (Wu, R.,
Grossman, L., and Moldave, K., eds.) Vol.101, pp. 20-51, Academic Press, New York
(3) For a book by one or more authors:
- Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning. A Laboratory
Manual pp. 1339-1341, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Text citations to references written by more than two authors should be styled for
example as, Smith et al. In the reference list, however, the names of all authors (with
initials) must be given. If an article has been accepted for publication by a journal but
has not yet appeared in print, the reference should be styled as follows:
- Tanahashi, H. and Ito, T. (1994) Molecular characterization of a novel factor
recognizing the interleukin-6 responsive element. J Biochem. (in press)
The use of "in preparation" and "submitted for publication" is not
allowed in the reference list.
6. Nucleotide Sequence
New nucleotide data must be submitted and deposited in the DDBJ/EMBL/GenBank databases and
an accession number obtained before the paper can be accepted for publication. Submission
to any one of the three collaborating databanks is sufficient to ensure data entry in all.
The accession number should be included in the manuscript e.g., as a footnote on the title
page: "Note: Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank
databases under the accession number(s) ....". If requested, the database will
withhold release of data until publication. The most convenient method for submitting
sequence data is by World Wide Web:
DDBJ via SAKURA: http://sakura.ddbj.nig.ac.jp/
EMBL via WEBIN: http://www.ebi.ac.uk/embl/Submission/webin.html
GenBankTM via BankIt: http://www.ncbi.nlm.nih.gov/BankIt/
or stand-alone submission tool
Sequin: http://www.ncbi.nlm.nih.gov/Sequin/
For special types of submissions (e.g., genomes, bulk submissions, etc.) additional
submission protocols are available from the above sites.
Database Contact Information
DDBJ: Center for Information Biology and DNA Data Bank of Japan
National Institute of Genetics, 1111 Yata, Mishima, Shizuoka 411-8540, JAPAN; telephone:
+81 559 81 6853; fax: +81 559 81 6849; e-mail: ddbj@ddbj.nig.ac.jp; web URL:
http://www.ddbj.nig.ac.jp/
EMBL: EMBL Nucleotide Sequence Submissions, European Bioinformatics Institute, Wellcome
Trust Genome Campus, Hinxton, Cambridge DB10 1SD U.K.; telephone: +44 1223 494499; fax:
+44 1223 494472; e-mail: datasubs@ebi.ac.uk; web URL: http://www.ebi.ac.uk
GenBank: National Center for Biotechnology Information, National Library of Medicine,
Bldg. 38A, Rm 8N-803, Bethesda, MD 20894, U.S.A.; telephone: +1 301 496 2475; fax: +1 301
480 9241; e-mail: info@ncbi.nlm.nih.gov; web URL: http://www.ncbi.nlm.nih.gov
7. Electronic manuscripts
Electronic manuscripts reduce the possibility of introducing errors and resulted in rapid
delivery of proofs. After acceptance, authors are encouraged to send the disk plus one
printed manuscript to the Editorial Office of the Journal.
V. PREPARATION OF TABLES
1. Tables should be drawn on separate sheets and
numbered consecutively in Roman numerals. For aid in designing tables in acceptable style,
refer to current issues of the Journal.
2. Each table should have an explanatory title and sufficient experimental
detail, usually in a paragraph immediately following the title, to be intelligible without
reference to the text (unless the procedure is given in the Experimental Procedures
section, or under another table or figure).
3. Indicate units of measure clearly.
4. Footnotes to tables should be kept to a minimum and should be indicated by
superscript lower cases, at the bottom of the table.
VI. PREPARATION OF ILLUSTRATIONS
1. Each figure (Scheme, Diagram) should be given on a
separate sheet numbered with an Arabic numeral (Fig. 1, Fig. 2, etc.). Figures will
be reduced to fit into the type area of the printed page (17.5 x 23.5 cm).
2. Identify all figures in the margin or on the back, with the author's name and
figure number and indicate TOP.
3. Each figure should be accompanied by a title and an explanatory legend
(Legends to Figures). There should be sufficient experimental detail in the legend to make
the figure intelligible without reference to the text (unless the same material has been
given with a previous figure, or in the Experimental Procedures section).
4. Legends to Figures should be typed double-spaced, in
numerical order, on a separate page.
5. Photographs should be glossy and as high in contrast as possible. Quadruplicate
copies for referees should be of the same quality as the original.
6. Indicate the magnification of photomicrographs in the legend or include a bar
indicating the scale in the figure.
7. Flow diagrams and amino acid or nucleotide sequences should always be
presented as direct photographic reproduction.
VII. CHEMICAL AND MATHEMATICAL FORMULAE
1. Refer in the text to simple chemical compounds by their formulae when these
can be printed in simple horizontal lines of type. Do not use structural formulae in the
running text.
2. Ionic charge should be shown as a superscript following the chemical symbol, e.g.
Fe3+, SO42.
3. Prepare large structural formulae and long mathematical equations in a form
suitable for direct photographic reproduction and include them as a Diagram at the end of
the paper.
4. Isotopically Labeled CompoundsThe symbol for an isotope is shown in
square brackets directly before the name (word), as in [14C]urea, [a-14C]leucine, DL-[methyl-14C]methionine. When
more than one position in a substance is labeled with the same isotope and the positions
are not indicated, the number of labeled atoms should be indicated as a right-hand
subscript; as in [14C2]glycolic acid. The symbol U indicates
uniform, e.g. [U-14C]glucose (where the 14C is
uniformly distributed among all six positions). The isotopic prefix precedes that part of
the name to which it refers, as in sodium [14C]formate, thiamine [b-32P]diphosphate. Terms such as 131I-labeled
albumin should not be contracted to [131I]albumin. When isotopes of more than
one element are introduced, their symbols should be arranged in alphabetical order: e.g.
L-[3-14C, 2,3-2H, 15N]serine. The symbols 2H
and 3H or D and T may be used for deuterium and tritium, respectively.
For simple molecules, the labeling is indicated by writing the chemical formulae with
the prefix superscripts attached to the correct atomic symbols in the formulae: e.g.
14CO2, H218O 2H2O. Square brackets
should not be used for them, or when the isotopic symbol is attached to a word that is not
a specific chemical name, abbreviation or symbol: e.g. 131I-labeled, 14C-sugar,
14C-steroids, 32PO43, but [32P]phosphate.
5. Spectrophotometric DataBeer's law may be stated as
A = log T = elc
Where A is the absorbance; T; the transmittance (=I/I0);
e;, the molar absorption coefficient; c, the
concentration of the absorbing substances in moles per liter; and l, the length of
the optical path in centimeters. Under these conditions e has
the dimensions litermol1cm1 or more briefly
M1cm1 (not cm2moll).
Do not use "O.D." and "E."
VIII. ETHICS
In scientific investigations involving human subjects, experiments should be
performed in accordance with the ethical standards formulated in the Helsinki Declaration
of 1964 (revised in 1989, cf. http://ohsr.od.nih.gov/).
Similarly, animal experiments should follow the ethical standards formulated in the
Helsinki Declaration, and measures taken to protect animals from pain or discomfort should
be mentioned.
IX. TERMINOLOGY AND
ABBREVIATIONS
1. Abbreviations with specific meanings may be used for convenience for
complex chemical substances, particularly in equations, tables, or figures. Avoid using
abbreviations in titles and summaries except the standard ones listed in Table II of Section IX-8.
2. Use abbreviations and symbols sparingly in the text. In chemical equations,
which traditionally depend upon symbols, an abbreviation or symbol may be used for a term
that appears in full in the neighboring text. Trivial names are usually sufficiently short
not to require abbreviations.
3. An abbreviated name or symbol in a column heading in a table, figure, or
photograph must either be taken from the "accepted" list given in Section IX-8
or formulated in accordance with the principles of Section IX-6.
4. For spelling of chemical names consult current issues of the Journal. For
chemical terms follow essentially the usages and rules recommended by International
Scientific Union, especially Nomenclature Committee of IUBMB (NC-IUBMB, IUBMB:
International Union of Biochemistry and Molecular Biology) and IUPAC-IUBMB Joint
Commission on Biochemical Nomenclature (JCBN, IUPAC: International Union of Pure and
Applied Chemistry): see the recommendations in Biochemical Nomenclature and Related
Documents (1978), available from The Biochemical Society, 7 Warwick Court, London WC1R
5DP, U.K. and in Biochemical Nomenclature and Related Documents. A Compendium, 2nd
edn (Liébecq, C., ed.), Portland Press Ltd, London (1992). (see Eur. J. Biochem. 213,
13 (1993))
Refer also to http://www.chem.qmw.ac.uk/iupac/jcbn/
5. EnzymesWhere one or more enzymes figure prominently in a manuscript,
authors should use the recommended (trivial) name or systematic name given by Nomenclature
Committee of IUBMB and IUPAC-IUBMB Commission on Biochemical Nomenclature: see
- Enzyme Nomenclature, Recommendations (1992), Academic Press Inc.,
- see also Eur. J. Biochem. 213, 13 (1993).
- Supplement Eur. J. Biochem. 223, 15 (1994).
- Supplement 2 Eur. J. Biochem. 232, 16 (1995).
- Supplement 3 Eur. J. Biochem. 237, 15 (1996).
- Supplement 4 Eur. J. Biochem. 250, 16 (1997).
When an enzyme is the main subject of a paper, its source, trivial name, systematic
name (or the reaction that it catalyzes) and code number (preceded by "EC")
should be included.
6. Non-Standard AbbreviationsUse of abbreviations other than the standard
ones listed in IX-7 and IX-8 should be kept to a minimum. Such abbreviations should be
introduced only when absolutely necessary, as in tables, figures, and other illustrations
where space is particularly limited. Abbreviations are usually not needed in the text of a
paper where repeated use of long names can be avoided by judicious use of pronouns, or by
paraphrasing with words such as "the substrate," "the inhibitor,"
"the methyl derivative" etc. All non-standard abbreviations used in
the text should be defined in alphabetical order in a single footnote on the title page.
7. Abbreviations of Units of Measurement and Physical and Chemical QuantitiesThese
abbreviations listed in Table I may be used without definition.
TABLE I
(1) Prefixes to the names of units
| tera |
1012 |
T |
milli |
103 |
m |
| giga |
109 |
G |
micro |
106 |
m |
| mega |
106 |
M |
nano |
109 |
n |
| kilo |
103 |
k |
pico |
1012 |
p |
| deci |
101 |
deci (not d) |
femto |
1015 |
f |
| centi |
102 |
c1) |
atto |
a |
(2) Units of Concentration2)
| molar (moles/liter) |
M |
| millimolar (millimoles/liter) |
mM (not 103 M) |
| micromolar (micromoles/liter) |
mM (or 106 M) |
| nanomolar (nanomoles/liter) |
nM (or x 109 M) |
| picomolar (picomoles/liter) |
pM (or x1012 M) |
(3) Units of Length
| meter |
m |
| centimeter |
cm |
| millimeter |
mm |
| micrometer (not micron) |
mm (not m) |
| nanometer |
nm (not mm) |
| Angstrom (0.1 nm) |
Å |
(4) Units of Area and Volume
| square centimeter |
cm2 |
| cubic centimeter |
cm3 |
| liter |
l (in table only) |
| milliliter |
ml |
| microliter |
ml (not l) |
(5) Units of Mass
| gram |
g (kg, mg, mg [not g], ng, pg) |
| dalton3) |
Da |
(6) Units of Time
| hour |
h |
year |
yr |
| minute |
m |
month |
mo |
| second |
s |
week |
wk |
|
|
day |
d |
(7) Units of Radioactivity
| becquerel |
Bq (= 1 dps or 60 dpm) |
| per minute |
Ci (= 3.7 x 1010 Bq) |
| curie(s) |
cpm |
| disintegrations per minute |
dpm |
(8) Other Units
| mole |
mol (mmol, mmol, nmol, pmol) |
| degree Celcius |
C |
| degree absolute (kelvin) |
K |
| joule |
J |
| kilojoule |
kJ |
| calorie |
cal |
| kilocalorie |
kcal |
| parts per billion |
ppb |
| parts per million |
ppm |
| cycles per second (hertz) |
Hz (not cps) |
| equivalent |
eq |
| ampere |
A (mA) |
| ohm |
W |
| volt |
V |
| gauss |
G |
| pascal |
Pa |
| revolutions per minute |
rpm |
Svedberg unit of
sedimentation coefficient (1013 s) |
S |
(9) Physical and Chemical Quantities
| absorbance |
A |
| equilibrium constant |
K |
| rate constant |
k |
| maximum velocity |
Vmax |
| Michaelis constant |
Km |
| equilibrium dissociation constant |
Kd |
| isoelectric point |
pI |
| molecular weight |
Mr |
| retardation factor |
Rf |
| acceleration of gravity |
g |
| specific rotation |
[a]tl |
| partial specific volume |
v (bar) |
| diffusion constant |
D |
| sedimentation coefficient |
s |
| density |
r |
sedimentation coefficientin water at 20C,
extraporated to zero concentration |
s020,w |
| Gibbs energy change |
DG |
| entropy change |
DS |
| enthalpy change |
DH |
| melting temperature |
Tm |
(10) Other Terms
| logarithm |
log |
| logarithm (natural) |
ln |
| standard deviation of a series |
SD |
| standard error of mean of series |
SE |
1) to be avoided where possible (except for cm).
2)Terms such as milligram percent (mg %) should not be
used. Weight concentrations should be given as g/ml, g/100 ml, etc.
3)Molecular weight is dimensionless. Only molecular mass
is expressed by daltons.
8. Accepted Abbreviations and SymbolsAuthors may use, without definition,
the abbreviations given in Table II and the symbols and abbreviations for amino acid or
nucleotide residues in polymers or sequences. Define other abbreviations in a single
footnote on the title page.
TABLE II
(1) General
| Adenosine 3':5'-cyclic monophosphate |
cAMP |
| Adenosine 5'-mono., di, and triphosphates1) |
AMP, ADP, and ATP |
| Adenosine triphosphatase |
ATPase |
| Base pair(s) |
bp |
| Bovine serum albumin |
BSA |
| O-(Carboxymethyl) |
CM- |
| Circular dichroism |
CD |
| Coenzyme A and its acyl derivatives |
CoA (or CoASH) and acyl-CoA |
| Complementary DNA |
cDNA |
| Cyclic AMP |
cAMP |
| Cyclic GMP |
cGMP |
| Cytidine diphosphate choline, etc. |
CDP-choline, etc. |
| Cytidine 5'-mono-, di-, and triphosphates |
CMP, CDP, and CTP |
| Deoxyribonuclease |
DNase |
| Deoxyribonucleic acid |
DNA |
| O-(Diethylaminoethyl) |
DEAE- |
| Dithiothreitol |
DTT |
| Electron paramagnetic resonance |
EPR |
| Electron spin resonance |
ESR |
| Ethylenediaminetetraacetic acid |
EDTA |
| [Ethylenebis(oxyethylenenitrilo)]-tetraacetic acid |
EGTA |
| Flavin-adenine dinucleotide and its fully reduced form |
FAD and FADH2 |
| Flavin mononucleotide and its fully reduced form |
FMN and FMNH2 |
| Fourier transform |
FT |
| Gas chromatography-mass spectrometry |
GC-MS |
| Gas liquid chromatography |
GLC |
| Glutathione and its oxidized form |
GSH and GSSG |
| Guanosine 3':5'-cyclic monophosphate |
cGMP |
| Guanosine 5'-mono-, di-, and triphosphates |
GMP, GDP, and GTP |
| Guanosine triphosphatase |
GTPase |
| Hemoglobin |
Hb |
| Heterogenous nuclear RNA |
hnRNA |
| High performance (pressure) liquid chromatography |
HPLC |
| (2-Hydroxyethyl)-1-piperazineethane-sulfonic acid |
HEPES |
| Immunoglobulin |
Ig (IgG, IgM, etc.) |
| Infrared |
IR |
| Inorganic orthophosphate |
Pi |
| Inorganic pyrophosphate |
PPi |
| Inosine 5'-mono-, di-, and triphosphates |
IMP, IDP, and ITP |
| Kilobases |
kb |
| Kilobase pairs |
kbp |
| Lethal dose, 500% |
LD50 |
| Messenger RNA |
mRNA |
| Nicotinamide adenine dinucleotide and its reduced form |
NAD+ and NADH2) |
| Nicotinamide adenine dinucleotide phosphate and its reduced form |
NADPH2) |
| Nuclear magnetic resonance |
NMR |
| Nuclear RNA |
nRNA |
| Optical rotatory dispersion |
ORD |
| Phosphoric acid residue |
P- or -P |
| Pseudouridine and pseudouridine mononucleotide |
y and yMP |
| Polyacrylamide gel electrophoresis |
PAGE |
| Poly(adenylic acid), polyadenylate3) |
Poly(A)3) |
| Polymerase chain reaction |
PCR |
| Restriction fragment length polymorphism |
RFLP |
|
| Ribonuclease |
RNase |
| Ribonucleic acid |
RNA |
| Ribosomal RNA |
rRNA |
| Ribosylthymine 5'-mono-, di-, and triphosphates |
TMP, TDP, and TTP |
| Sodium dodecyl sulfate |
SDS |
| Thin layer chromatography |
TLC |
| Thymidine (2'-deoxyribosylthymine) 5'-mono-, di-, and triphosphates |
dTMP, dTDP, and dTTP4) |
| Transfer RNA |
tRNA |
| Tris(hydroxymethyl)aminomethane |
Tris |
| Ultraviolet |
UV |
| Uridine diphosphate glucose, etc. |
UDP-glucose, etc. |
| Uridine 5'-mono-, di-, and triphosphates |
UMP, UDP, and UTP |
(2) Amino acids Alanine Arginine Asparagine
| Alanine |
Ala (A) |
| Arginine |
Arg (R) |
| Asparagine |
Asn (N) |
| Aspartic acid |
Asp (D) |
| Aspartic acid or asparagine |
Asx (B) |
| Cysteine |
Cys (C) |
| Glutamic acid |
Glu (E) |
| Glutamine |
Gln (Q) |
| Glutamic acid or glutamine |
Glx (Z) |
|
| Glycine |
Gly (G) |
| Histidine |
His (H) |
| Isoleucine |
Ile (I) |
| Leucine |
Leu (L) |
| Lysine |
Lys (K) |
| Methionine |
Met (M) |
| Phenylalanine |
Phe (F) |
| Proline |
Pro (P) |
| Serine |
Ser (S) |
| Threonine |
Thr (T) |
| Tryptophan |
Trp (W) |
| Tyrosine |
Tyr (Y) |
| Valine |
Val (V) |
|
(3) Nucleic acids
| Adenosine |
A |
| Bromouridine |
BrUrd or B |
| Cytidine |
C |
| Dihydrouridine |
D or hU |
| Guanosine |
G |
| Inosine |
I |
| 6-Mercaptopurine ribonucleoside (6-thioinosine) |
M or sI |
| 'a nucleoside' |
Nuc or N |
| Pseudouridine |
y or Qa |
| 'a purine nucleoside' |
R |
| 'a pyrimidine nucleoside' |
Y |
| Thiouridine |
S or sU |
| Thymidine (2'-deoxyribosylthymine) |
dT |
| Uridine |
U |
| Xanthosine |
X |
| Phosphoric residue |
-P or p |
l) The various isomers of adenosine monophosphate may
be written 2'-AMP, 3'-AMP, or 5'-AMP (in case of possible ambiguity). A similar procedure
may be applied to other nucleoside or deoxyribonucleoside monophosphates.
2)NAD(P)+ and NAD(P)H indicate either NAD+
or NADP+ and either NADH or NADPH, respectively.
3)Similarly abbreviate oligo- and polynucleotides
composed of repeating sequences or of unknown sequence of given purine or pyrimidine
bases, e.g. oligothymidylate, oligo(dT); alternating copolymer of A and U,
poly(A-U); random copolymer of A and U, poly(A,U).
4)The d prefix may be used to represent the
corresponding deoxyribonucleoside phosphates, e.g. dADP.
9. Names of Animals, Plants, and MicroorganismsThe scientific names are
Latin binomials and should be given in full in the title and summary and on first mention
in the text (e.g. Escherichia coli). Subsequently, the generic name may be
contracted (usually to the first letter), e.g., E. coli. The strain of laboratory
animals and if possible the source should be stated.
10. The cytochromes should be designated by a small italicized letter, e.g.
cytochrome a, b2, c1, etc.
X. COPYRIGHT
The Journal if copyrighted by the Japanese Biochemical Society. Requests for any
reproduction or translation of this journal should be made in duplicate to the Managing
Editor of the Journal,
The Japanese Biochemical Society
Ishikawa Building-3f
25-16, Hongo 5-chome, Bunkyo-ku
Tokyo 113-0033, Japan
and should include a statement of intended use as well as explicit specifications of
the material to be reproduced.
Authors are advised to consult "Notes to Contributors" appearing in every
issue of the Journal in which any revisions of "Instructions to Authors" will be
announced.
XI. CHARGES TO AUTHORS
A page charge (currently 700 yen and 7,200 yen per printed page of a Regular Paper
and a Rapid Communication, respectively, and subject to change) is levied to help
publication cost. Under exceptional circumstances, when no source of grant or other
support exists, the authors may apply, at the time of submission, for a grant-in-aid to
the Editor-in-Chief.
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